RINNIR Beyond5G FIRST Konan Univ.


Publications including proceedings are HERE (Japanese page)
Presentations are HERE (Japanese page)


    2025

  1. Synthesis of biocompatible gold nanoparticles for photothermal therapy by mineralization using peptides
    Shuhei Yoshida, Koki Yoshida, Yoshiki Shitamukai, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui*
    RSC Advances, 15, 48521-48528
    2025/12/08
    DOI: 10.1039/d5ra08717a
    [journal page]

  2. One-Pot Synthesis and Immobilization of Gold Nanoparticles Using Peptidyl Microbeads
    Shuhei Yoshida, Koki Yoshida, Taichi Isozaki, Maho Oura, Makoto Ozaki, Takaaki Tsuruoka, Kenji Usui*
    Molecules, 30, 1689
    2025/04/10
    [journal page] [Pubmed]

  3. Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads
    Shuhei Yoshida, Koki Yoshida, Yoshio Hamada, Takaaki Tsuruoka, Kenji Usui*
    Scientific Reports, 15, 3249
    2025/01/25
    [journal page] [Pubmed]

  4. Complex and Non-sequential Current Signatures of a β-Hairpin Peptide Confined in a Nanopore
    Misa Yamaji, Mauro Chinappi, Blasco Morozzo della Rocca, Kenji Usui, Ryuji Kawano
    Analytical Chemistry, 97, 2044-2051
    2025/01/22
    DOI: 10.1021/acs.analchem.4c04150
    [journal page] [Pubmed]

    5. TOP


    2024

  5. Development of a CaCO3 Precipitation Method Using a Peptide and Microwaves Generated by a Magnetron
    Fumihiro Kayamori, Hiroyuki Togashi, Natsumi Endo, Makoto Ozaki, Kan Hirao, Yonejiro Arimoto, Ryuji Osawa, Takaaki Tsuruoka, Takahito Imai, Kin-ya Tomizaki, Tomohiro Umetani, Nobuhiro Nakanishi, Kenji Usui*
    Processes, 12, 1327
    2024
    DOI: 10.3390/pr12071327
    [Journal page]

    6. TOP


    2023

  6. Shape control of Au nanostructures using peptides for biotechnological applications
    Shuhei Yoshida, Kin-ya Tomizaki, Kenji Usui*
    Chemical Communications, 59, 13239-13244 ※Back cover feature
    2023/11/07
    DOI: 10.1039/d3cc04331b
    [Journal page] [Pubmed] [Back cover]

  7. Effect of linearly polarized microwaves on nanomorphology of calcium carbonate mineralization using peptides
    Kenji Usui*, Makoto Ozaki, Kan Hirao, Tsubasa Kosaka, Natsumi Endo, Shuhei Yoshida, Shin-ichiro Yokota, Yonejiro Arimoto, Ryuji Osawa, Nobuhiro Nakanishi, Kin-ya Tomizaki, Tomohiro Umetani, Fumihiro Kayamori*
    Scientific Reports, 13, 12027
    2023/07/13
    DOI: 10.1038/s41598-023-37473-7
    [Journal page] [Pubmed]

    8. TOP


    2022

  8. De novo design of a nanopore for single-molecule detection that incorporates a beta-hairpin peptide
    Keisuke Shimizu, Batsaikhan Mijiddorj, Masataka Usami, Ikuro Mizoguchi, Shuhei Yoshida, Shiori Akayama, Yoshio Hamada, Akifumi Ohyama, Kenji Usui, Izuru Kawamura, Ryuji Kawano
    Nature Nanotechnology, 17, 67-75
    2022/01
    [Journal page] [Pubmed]

    9. TOP


    2021

  9. Intracellular Mineralization of Gold Nanoparticles Using Gold Ion-Binding Peptides with Cell-Penetrating Ability
    Makoto Ozaki, Shuhei Yoshida, Takaaki Tsuruoka, Kenji Usui*
    Chemical Communications, 57, 725-728 ※Back cover feature
    2021/01
    DOI: 10.1039/D0CC06117D
    [Journal page] [Pubmed] [Back cover]

  10. Elemental Composition Control of Gold-Titania Nanocomposites by Site-Specific Mineralization Using Artificial Peptides and DNA
    Makoto Ozaki, Takahito Imai, Takaaki Tsuruoka, Shungo Sakashita, Kin-ya Tomizaki, Kenji Usui*
    Communications Chemistry, 4, 1
    2021/01
    DOI: 10.1038/s42004-020-00440-8
    [Journal page]

    11. TOP


    2020

  11. Effect of Tryptophan Residues on Gold Mineralization by a Gold Reducing Peptide
    Makoto Ozaki, Shuhei Yoshida, Maho Oura, Takaaki Tsuruoka, Kenji Usui*
    RSC Advances, 10, 40461-40466
    2020/11
    DOI: 10.1039/D0RA07098J
    [Journal page]

  12. Mass Spectrometry-Based Solid Phase Peptide Reaction Assay for Detecting Allergenicity Using an Immobilized Peptide-Conjugating Photo-Cleavable Linker
    Hiroshi Miyazaki,Yasutaka Samejima, Kazuya Iwata, Yuuki Minamino, Shinya Hikida, Hideto Ariumi, Hidefumi Ikeda, Yoshio Hamada, Kunihiko Yamashita, Kenji Usui*
    International Journal of Molecular Sciences, 21, 8332
    2020/11
    DOI: 10.3390/ijms21218332
    [Journal page]

  13. Synthesis of Peptide-Immobilized Magnetic Beads, and Peptide Reactivity Assay for Assessing Skin Sensitization Utilizing Chromophore
    Hiroshi Miyazaki, Hikaru Takaishi, Hidefumi Ikeda, Hideto Ariumi, Yoshio Hamada, Kunihiko Yamashita, Kenji Usui*
    Processes, 8, 1257
    2020/09
    DOI: 10.3390/pr8101257
    [Journal page]

  14. Novel Purification Process for Amyloid Beta Peptide(1-40)
    Kenji Usui*,Shin-ichiro Yokota, Kazuya Iwata, Yoshio Hamada
    Processes, 8, 464 ※Cover feature
    2020/04
    DOI: 10.3390/pr8040464
    [Journal page] [Cover Story] [Cover]

  15. Development of a Chromophore-Solid Phase Peptide Reaction Assay (C-SPRA) for Assessing Skin Sensitization In Vitro
    Hiroshi Miyazaki, Yoshio Hamada, Hikaru Takaishi, Yuuki Minamino, Hidefumi Ikeda, Hideaki Mekata, Masayuki Takaishi, Kunihiko Yamashita, Kenji Usui*
    Analyst, 145, 3211-3216 ※Inside back cover feature
    2020/03
    DOI: 10.1039/C9AN02514F
    [Journal page] [Pubmed] [Inside back cover]

    16. TOP


    2018

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    2017

  16. DNA G-Wire Formation Using an Artificial Peptide is Controlled by Protease Activity
    Kenji Usui*, Arisa Okada, Shungo Sakashita, Masayuki Shimooka, Takaaki Tsuruoka, Shu-ichi Nakano, Daisuke Miyoshi, Tsukasa Mashima, Masato Katahira, Yoshio Hamada
    Molecules, 22, 1991
    2017/11
    DOI: 10.3390/molecules22111991
    [Journal page] [PubMed]

  17. Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier
    Kin-ya Tomizaki*, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui*
    Molecules, 22, 1916
    2017/11
    DOI: 10.3390/molecules22111916
    [Journal page] [PubMed]

  18. Fluorescent and Luminescent Fusion Proteins for Analyses of Amyloid Beta Peptide Aggregation.
    Kenji Usui, Masayasu Mie, Takashi Andou, Hisakazu Mihara, Eiry Kobatake
    J Pept Sci, 23, 659-665
    2017/07
    DOI: 10.1002/psc.3003
    [Journal page] [PubMed]

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    2016

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    2015

  19. Label and Label-Free Detection Techniques for Protein Microarrays
    Amir Syahir, Kenji Usui*, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara
    Microarrays, 4, 228-244
    DOI: 10.3390/microarrays4020228
    2015/04
    [Journal page] [PubMed]

  20. Control of Guanine-Rich DNA Secondary Structures Depending on the Protease Activity Using a Designed PNA Peptide
    Kenji Usui*, Arisa Okada, Keita Kobayashi, Naoki Sugimoto
    Org. Biomol. Chem., 13, 2022-2025
    DOI: 10.1039/c4ob02535k
    2015/02
    [Journal page] [PubMed]

  21. ATP-Binding Peptide-Hydrogel Composite Synthesized by Molecular Imprinting on Beads
    Ayana Takata, Kenji Usui, Jun Matsui
    Molecular Imprinting, 3, 65-70
    [Journal page]

    22. TOP


    2014

  22. Cellular Differentiation Assessments by Measuring the Degree of Cellular Internalization and Membrane Adsorption Using Designed Peptides.
    Kenji Usui*, Takuya Kikuchi, Kunio Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Bioorg. Med. Chem. lett., 24, 4129-4131
    DOI: 10.1016/j.bmcl.2014.07.053
    2014/09
    [Journal page] [PubMed]

  23. Peptides Targeting G-Quadruplex Structures
    Arisa Okada, Kenji Usui*
    Chemical Biology of Nucleic Acids:Fundamentals and Clinical Applications, 459-475
    [Journal page]

    24. TOP


    2013

  24. A Novel Array Format for Monitoring Cellular Uptake Using a Photo-Cleavable Linker for Peptide Release
    Kenji Usui*, Takuya. Kikuchi, Kin-ya Tomizaki, Takashi Kakiyama, Hisakazu Mihara
    Chem. Commun., 49, 6394-6396
    DOI: 10.1039/c3cc41632a
    2013/07
    [Journal page] [PubMed]

  25. Systematic Screening of the Cellular Uptake of Designed Alpha-Helix Peptides
    Kenji Usui*, Takuya. Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Bioorg. Med. Chem., 21, 2560-2567
    DOI: 10.1016/j.bmc.2013.02.030
    2013/05
    [Journal page] [PubMed]

  26. A Peptide Release System Using a Photo-Cleavable Linker in a Cell Array Format for Cell-Toxicity Analysis
    Takashi Kakiyama, Kenji Usui*, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Polym J., 45, 535-539
    [Journal page]

    27. TOP


    2011

  27. Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
    Keita Kobayashi, Noriko Matsui, Kenji Usui*
    J. Nucleic Acids , 2011:572873
    DOI: 10.4061/2011/572873
    [Journal page] [PubMed]

  28. Cell Fingerprint Patterns Using Designed α-Helical Peptides to Screen for Cell-Specific Toxicity
    Kenji Usui, Takashi Kakiyama, Kin-ya Tomizaki, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
    Bioorg. Med. Chem. Lett., 21, 6281-6284
    DOI: 10.1016/j.bmcl.2011.09.002
    2011/11
    [Journal page] [PubMed]

  29. Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone H3-Mimicking Peptide
    Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsui, Daisuke Miyoshi, Naoki Sugimoto
    Chem. Commun., 47, 3967-3971
    DOI: 10.1039/c0cc05776b
    2011/03
    [Journal page] [PubMed]

    30. TOP


    2010

  30. Protein-protein Interactions and Selection: Array-Based Techniques for Screening Disease-Associated Biomarkers in Predictive/Early Diagnosis
    Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    FEBS J., 277, 1996-2005
    DOI: 10.1111/j.1742-4658.2010.07626.x.
    2010/05
    [Journal page] [PDF] [PubMed]

    31. TOP


    2009

  31. Site-Specific Modification of Alzheimer's Peptides by Cholesterol Oxidation Products Enhances Aggregation Energetics and Neurotoxicity
    Kenji Usui, John D. Hulleman, Johan F. Paulsson, Sarah J. Siegel, Evan T. Powers, Jeffery W. Kelly
    Proc. Natl. Acad. Sci. USA, 106, 18563-18568
    DOI: 10.1073/pnas.0804758106
    2009/11
    [Journal page] [PDF] [PubMed]

  32. A Designed Peptide Chip: Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining
    Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
    Methods. Mol. Biol., 570, 273-284
    DOI: 10.1007/978-1-60327-394-7_13
    [Journal page] [PubMed]

  33. Proteins: Array-Based Techniques
    Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    Wiley Encyclopedia of Chemical Biology, 4, 144-158
    DOI: 10.1002/9780470048672.wecb022
    [Journal page]

    34. TOP


    2007

  34. Screening of Alpha-Helical Peptide Ligands Controlling a Calcineurin-Phosphatase Activity
    Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
    Bioorg. Med. Chem. Lett., 17, 167-171
    DOI: 10.1016/j.bmcl.2006.09.075
    2007/01
    [Journal page] [PubMed]

  35. Interactions between Peptides Containing Nucleobase Amino Acids and T7 Phages Displaying S. Cerevisiae Proteins
    Sinya Watanabe, Kin-ya Tomizaki, Tsuyoshi Takahashi, Kenji Usui, Kotaro Kajikawa, Hisakazu Mihara
    Biopolymers, 88, 131-140
    DOI: 10.1002/bip.20662
    [Journal page] [PubMed]

    36. TOP


    2006

  36. Protein-Fingerprint Data Mining of a Designed Alpha-Helical Peptide Array
    Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
    Mol. BioSyst., 2, 417-420 ※Inside back cover feature
    2006/09
    [Journal page] [PubMed]

  37. A Novel Peptide Microarray for Protein Detection and Analysis Utilizing a Dry Peptide Array System
    Kenji Usui, Kin-ya Tomizaki, Takafumi Ohyama, Kiyoshi Nokihara, Hisakazu Mihara
    Mol. BioSyst., 2, 113-121
    DOI: 10.1039/b514263f
    2006/02
    [Journal page] [PubMed]

  38. A PNA-DNA Hybridization Chip Approach for the Detection of Beta-Secretase Activity
    Shusuke Sano, Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    Bioorg. Med. Chem. Lett., 16, 503-506
    DOI: 10.1016/j.bmcl.2005.10.064
    2006/02
    [Journal page] [PubMed]

    39. TOP


    2005

  39. A Designed Glycopeptide Array for Characterization of Sugar-Binding Proteins toward a Glycopeptide Chip Technology
    Kenji Usui, Tetsunori Ojima, Kin-ya Tomizaki, Hisakazu Mihara
    Nanobiotechnology, 1, 191-200
    [Journal page]

  40. IR Study on Stacking Manner of Peptide Nanorings in Peptide Nanotubes
    Yukiko Nagai, Tsutomu Nakanishi, Hajime Okamoto, Kyozaburo Takeda, Yukio Furukawa, Kenji Usui, Hisakazu Mihara
    Jpn. J. Appl. Phys., 44, 7654-7661
    [Journal page]

  41. Difference in Self-Assembling Morphology of Peptide Nanorings
    Hajime Okamoto, Tetsuo Yamada, Hiroshi Miyazaki, Tsutomu Nakanishi, Kyozaburo Takeda, Kenji Usui,Ikuo Obataya, Hisakazu Mihara, Hiroaki Azehara, Wataru Mizutani, Katsushi Hashimoto, Hiroshi Yamaguchi, Yoshiro Hirayama
    Jpn. J. Appl. Phys., 44, 8240-8248
    [Journal page]

  42. Anomalous Reflection of Gold Applicable for a Practical Protein-Detecting Chip Platform
    Sinya Watanabe, Kenji Usui, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara
    Mol. BioSyst., 1, 363-365
    DOI: 10.1039/b513075c
    2005/12
    [Journal page] [PubMed]

  43. Protein-Detecting Microarrays: Current Accomplishments and Requirements
    Kin-ya Tomizaki, Kenji Usui, Hisakazu Mihara
    ChemBioChem, 6, 782-799 ※Inside back cover feature
    DOI: 10.1002/cbic.200400232
    2005/05
    [Journal page] [PubMed]

    44. TOP


    2004

  44. Peptide Arrays with Designed Alpha-Helical Structures for Characterization of Proteins from FRET-Fingerprint Patterns
    Kenji Usui, Mizuki Takahashi, Kiyoshi Nokihara, Hisakazu Mihara
    Mol. Divers., 8, 209-218
    [Journal page] [PubMed]

  45. Peptide Arrays with Designed Secondary Structures for Protein Characterization Using Fluorescent Fingerprint Patterns
    Kenji Usui, Tetsunori Ojima, Mizuki Takahashi, Kiyoshi Nokihara, Hisakazu Mihara
    Biopolymers, 76, 129-139
    DOI: 10.1002/bip.10568
    [Journal page] [PubMed]

  46. Development of Peptide-Chips Focusing on a High Throughput Protein-Detection System
    Kiyoshi Nokihara, Takafumi Ohyama, Kenji Usui, Koichi. Yoneyama, Mizuki Takahashi, Hisakazu Mihara
    Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries, 83-88

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